Title | Accelerated solvent extraction of red onion peel extract and its antimicrobial, antibiofilm, and quorum-sensing inhibition activities against Listeria monocytogenes and Chromobacterium violaceum |
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ID_Doc | 10716 |
Authors | Dhowlaghar, N; Dhanani, T; Pillai, SS; Patil, BS |
Title | Accelerated solvent extraction of red onion peel extract and its antimicrobial, antibiofilm, and quorum-sensing inhibition activities against Listeria monocytogenes and Chromobacterium violaceum |
Year | 2023 |
Published | |
Abstract | In this study, we used accelerated solvent extraction (ASE) to extract flavonoids and anthocyanins from dried red onion peels, producing red onion peel extract (ROPE). High-performance liquid chromatography analysis of ROPE obtained using ASE identified important flavonols and anthocyanins. Total flavonoid and anthocyanin contents measured from ROPE were 459.15 mg/g +/- 16.45 (DW) and 33.14 mg/g +/- 0.99 (DW), respectively. ROPE antimicrobial activity against L. monocytogenes showed strain-specific effects, where the minimum inhibitory concentration (MIC) for V7 was two-fold greater than for EGD and Scott A (i.e., 25.82 vs. 12.91 mg quercetin-3, 4 '-diglucoside equivalents/gram [mg QdGE/g] ROPE). ROPE significantly inhibited (P < 0.05) biofilm formation by all three strains at sub-inhibitory concentrations (0.40-6.45 mg QdGE/g) in a concentration-dependent manner. Pre-formed biofilms of mixed L. monocytogenes strains on stainless steel were inactivated after 48 h at 22 |
http://manuscript.elsevier.com/S2212429223003000/pdf/S2212429223003000.pdf |
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