| Title | A model-driven approach to upcycling recalcitrant feedstocks in Pseudomonas putida by decoupling PHA production from nutrient limitation |
|---|---|
| ID_Doc | 12769 |
| Authors | Manoli, MT; Gargantilla-Becerra, A; Sánchez, CD; Rivero-Buceta, V; Prieto, MA; Nogales, J |
| Title | A model-driven approach to upcycling recalcitrant feedstocks in Pseudomonas putida by decoupling PHA production from nutrient limitation |
| Year | 2024 |
| Published | Cell Reports, 43.0, 4 |
| Abstract | Bacterial polyhydroxyalkanoates (PHAs) have emerged as promising eco-friendly alternatives to petroleumbased plastics since they are synthesized from renewable resources and offer exceptional properties. However, their production is limited to the stationary growth phase under nutrient -limited conditions, requiring customized strategies and costly two-phase bioprocesses. In this study, we tackle these challenges by employing a model -driven approach to reroute carbon flux and remove regulatory constraints using synthetic biology. We construct a collection of Pseudomonas putida -overproducing strains at the expense of plastics and lignin -related compounds using growth -coupling approaches. PHA production was successfully achieved during growth phase, resulting in the production of up to 46% PHA/cell dry weight while maintaining a balanced carbon -to -nitrogen ratio. Our strains are additionally validated under an upcycling scenario using enzymatically hydrolyzed polyethylene terephthalate as a feedstock. These findings have the potential to revolutionize PHA production and address the global plastic crisis by overcoming the complexities of traditional PHA production bioprocesses. |
| http://www.cell.com/article/S2211124724003073/pdf |
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