| Title | Extraction and Characterization of Collagen from Elasmobranch Byproducts for Potential Biomaterial Use |
|---|---|
| ID_Doc | 15131 |
| Authors | Seixas, MJ; Martins, E; Reis, RL; Silva, TH |
| Title | Extraction and Characterization of Collagen from Elasmobranch Byproducts for Potential Biomaterial Use |
| Year | 2020 |
| Published | Marine Drugs, 18, 12 |
| Abstract | With the worldwide increase of fisheries, fish wastes have had a similar increase, alternatively they can be seen as a source of novel substances for the improvement of society's wellbeing. Elasmobranchs are a subclass fished in high amounts, with some species being mainly bycatch. They possess an endoskeleton composed mainly by cartilage, from which chondroitin sulfate is currently obtained. Their use as a viable source for extraction of type II collagen has been hypothesized with the envisaging of a biomedical application, namely in biomaterials production. In the present work, raw cartilage from shark (Prionace glauca) and ray (Zeachara chilensis and Bathyraja brachyurops) was obtained from a fish processing company and submitted to acidic and enzymatic extractions, to produce acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). From all the extractions, P. glauca PSC had the highest yield (3.5%), followed by ray ASC (0.92%), ray PSC (0.50%), and P. glauca ASC (0.15%). All the extracts showed similar properties, with the SDS-PAGE profiles being compatible with the presence of both type I and type II collagens. Moreover, the collagen extracts exhibited the competence to maintain their conformation at human basal temperature, presenting a denaturation temperature higher than 37 degrees C. Hydrogels were produced using P. glauca PSC combined with shark chondroitin sulfate, with the objective of mimicking the human cartilage extracellular matrix. These hydrogels were cohesive and structurally-stable at 37 degrees C, with rheological measurements exhibiting a conformation of an elastic solid when submitted to shear strain with a frequency up to 4 Hz. This work revealed a sustainable strategy for the valorization of fisheries' by-products, within the concept of a circular economy, consisting of the use of P. glauca, Z. chilensis, and B. brachyurops cartilage for the extraction of collagen, which would be further employed in the development of hydrogels as a proof of concept of its biotechnological potential, ultimately envisaging its use in marine biomaterials to regenerate damaged cartilaginous tissues. |
| https://www.mdpi.com/1660-3397/18/12/617/pdf |
Similar Articles
| ID | Score | Article |
|---|---|---|
15189
|
Bisht, M; Martins, M; Dias, ACRV; Ventura, SPM; Coutinho, JAP Uncovering the potential of aqueous solutions of deep eutectic solvents on the extraction and purification of collagen type I from Atlantic codfish (Gadus morhua)(2021)Green Chemistry, 23, 22 | |
| 10628
|
Prajaputra, V; Isnaini, N; Maryam, S; Ernawati, E; Deliana, F; Haridhi, HA; Fadli, N; Karina, S; Agustina, S; Nurfadillah, N; Arisa, II; Desiyana, LS; Bakri, TK Exploring marine collagen: Sustainable sourcing, extraction methods, and cosmetic applications(2024) | |
| 25202
|
Martins, E; Fernandes, R; Alves, AL; Sousa, RO; Reis, RL; Silva, TH Skin Byproducts of Reinhardtius hippoglossoides (Greenland Halibut) as Ecosustainable Source of Marine Collagen(2022)Applied Sciences-Basel, 12, 21 | |
| 10664
|
Martins, E; Reis, RL; Silva, TH In Vivo Skin Hydrating Efficacy of Fish Collagen from Greenland Halibut as a High-Value Active Ingredient for Cosmetic Applications(2023)Marine Drugs, 21, 2 | |
| 13407
|
Cutajar, N; Lia, F; Deidun, A; Galdies, J; Arizza, V; Mangion, MZ Turning Waste into A Resource: Isolation and Characterization of High-Quality Collagen and Oils from Atlantic Bluefin Tuna Discards(2022)Applied Sciences-Basel, 12, 3 | |
| 21031
|
Roncoroni, M; Martinelli, G; Farris, S; Marzorati, S; Sugni, M Sea Urchin Food Waste into Bioactives: Collagen and Polyhydroxynaphtoquinones from P. lividus and S. granularis(2024)Marine Drugs, 22, 4 | |
| 9564
|
Grasso, F; Paz, DM; Sobrado, RV; Orlandi, V; Turrini, F; Agostinis, L; Morandini, A; Jenssen, M; Lian, KR; Boggia, R Feasibility of Enzymatic Protein Extraction from a Dehydrated Fish Biomass Obtained from Unsorted Canned Yellowfin Tuna Side Streams: Part II(2024)Gels, 10.0, 4 | |
| 12204
|
Mutalipassi, M; Esposito, R; Ruocco, N; Viel, T; Costantini, M; Zupo, V Bioactive Compounds of Nutraceutical Value from Fishery and Aquaculture Discards(2021)Foods, 10.0, 7 | |
| 29278
|
Melotti, L; Martinello, T; Perazzi, A; Iacopetti, I; Ferrario, C; Sugni, M; Sacchetto, R; Patruno, M A Prototype Skin Substitute, Made of Recycled Marine Collagen, Improves the Skin Regeneration of Sheep(2021)Animals, 11.0, 5 | |
| 10054
|
Valcarcel, J; Fraguas, J; Hermida-Merino, C; Hermida-Merino, D; Piñeiro, MM; Vázquez, JA Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities(2021)Marine Drugs, 19.0, 9 |