| Title |
Extraction, purification and in vitro assessment of the antioxidant and anti-inflammatory activity of policosanols from non-psychoactive Cannabis sativa L. |
| ID_Doc |
15880 |
| Authors |
Caroli, C; Baron, G; Cappellucci, G; Brighenti, V; Della Vedova, L; Fraulini, F; Oliaro-Bosso, S; Alessandrini, A; Zambon, A; Lusvardi, G; Aldini, G; Biagi, M; Corsi, L; Pellati, F |
| Title |
Extraction, purification and in vitro assessment of the antioxidant and anti-inflammatory activity of policosanols from non-psychoactive Cannabis sativa L. |
| Year |
2024 |
| Published |
Heliyon, 10, 9 |
| Abstract |
Policosanols (PCs) are bioactive compounds extracted from different natural waxes. In this work, the purification, characterization and assessment of the antioxidant and anti-inflammatory activity was carried out on PCs from an innovative source, i.e. a waxy material from supercriticalfluid extraction (SFE) of non -psychoactive Cannabis sativa L. (hemp) inflorescences. Starting from this material, PCs were obtained by microwave -assisted trans -esterification and hydrolysis, followed by preparative liquid chromatography under normal phase conditions. The purified product was characterized using high-performance liquid chromatography (HPLC) with an evaporative light scattering detector (ELSD). In vitro cell -free and cell -based antioxidant and antiinflammatory assays were then performed to assess their bioactivity. |
| PDF |
https://doi.org/10.1016/j.heliyon.2024.e30291
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