| Title |
Enrichment and application of extracellular nonulosonic acids containing polymers of Accumulibacter |
| ID_Doc |
18139 |
| Authors |
Tomás-Martínez, S; Chen, L; Pabst, M; Weissbrodt, DG; van Loosdrecht, MCM; Lin, YM |
| Title |
Enrichment and application of extracellular nonulosonic acids containing polymers of Accumulibacter |
| Year |
2023 |
| Published |
Applied Microbiology And Biotechnology, 107.0, 2-3 |
| Abstract |
Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that "Candidatus Accumulibacter " can produce Pse or Leg analogues as part of its extracellular polymeric substances (EPS). In order to employ a "Ca. Accumulibacter " enrichment as production platform for bacterial sialic acids, it is necessary to determine which fractions of the EPS of "Ca. Accumulibacter " contain NulOs and how to enrich and/or isolate them. We extracted the EPS from granules enriched with "Ca. Accumulibcater " and used size-exclusion chromatography (SEC) to separate them into different molecular weight (MW) fractions. This separation resulted in two high molecular weight (> 5500 kDa) fractions dominated by polysaccharides, with a NulO content up to 4 times higher than the extracted EPS. This suggests that NulOs in "Ca. Accumulibacter " are likely located in high molecular weight polysaccharides. Additionally, it was seen that the extracted EPS and the NulO-rich fractions can bind and neutralize histones. This opens the possibility of EPS and NulO-rich fractions as potential source for sepsis treatment drugs. |
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