| Title | Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production |
|---|---|
| ID_Doc | 18759 |
| Authors | Fontes, A; Francisco, R; Ferreira, FC; Faria, NT; Marques, S; Reis, A; Moura, P; Lukasik, R; Santos, J; da Silva, TL |
| Title | Monitoring Yeast Cultures Grown on Corn Stover Hydrolysate for Lipid Production |
| Year | 2024 |
| Published | Processes, 12.0, 3 |
| Abstract | Microbial oils can be used as an alternative sustainable and renewable feedstock to fossil reserves for producing lubricants and polyurethane materials. Two oleaginous yeasts were grown on non-detoxified corn stover hydrolysate supplemented with corn steep liquor and mineral medium in shake flasks. Trichosporon oleaginosus DSM 11815 displayed the highest lipid production. This strain was further cultivated in a bench bioreactor, using the same culture medium, under a batch regime. Flow cytometry was used to monitor the T. oleaginosus culture using the dual staining technique (SYBR Green and PI) for cell membrane integrity detection. Values of 42.28% (w/w) and 0.06 g/Lh lipid content and lipid productivity, respectively, were recorded for T. oleaginosus cultivated in the bench bioreactor operated under a batch regime. During the cultivation, most of the yeast cells maintained their integrity. T. oleaginosus has the potential to be used as an oil microbial source for a wide range of industrial applications. In addition, it is robust in adverse conditions such as lignocellulosic hydrolysate exposure and oxygen-limiting conditions. Flow cytometry is a powerful and useful tool for monitoring yeast cultivations on lignocellulosic hydrolysates for cell count, size, granularity, and membrane integrity detection. |
| https://www.mdpi.com/2227-9717/12/3/558/pdf?version=1710302559 |
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