| Title |
Eco-Circular Production of Demineralized Bioactive Peptides from Bovine Hemoglobin by Performing the Necessary Steps Simultaneously Using Bipolar Membrane Electrodialysis |
| ID_Doc |
21739 |
| Authors |
Abou-Diab, M; Thibodeau, J; Fliss, I; Dhulster, P; Nedjar, N; Bazinet, L |
| Title |
Eco-Circular Production of Demineralized Bioactive Peptides from Bovine Hemoglobin by Performing the Necessary Steps Simultaneously Using Bipolar Membrane Electrodialysis |
| Year |
2021 |
| Published |
Acs Sustainable Chemistry & Engineering, 9.0, 50 |
| Abstract |
There is great interest in peptides derived from bovine hemoglobin, a protein from slaughterhouse waste, as they have been demonstrated as potential novel class of natural conservative agents. The aim of the present study was to test a new original electrodialysis with a bipolar membrane (EDBM) configuration, allowing the simultaneous denaturation of hemoglobin, inactivation of pepsin, and demineralization of the hydrolysates, to produce bioactive peptides without adding chemicals and avoiding membrane fouling. Until now, this ecofriendly process has never been achieved without fouling. An integrated "3-in-1" application using an EDBM system forming four compartments (including the electrolyte compartment) was carried out and compared with conventional hydrolysis. The EDBM method was efficient for denaturing hemoglobin, inactivating the enzymatic reaction, and allowing a demineralization rate of 85% of hemoglobin hydrolysate. However, fouling was still observed on the anionic membrane because of hem precipitation. To avoid fouling, an additional step of discoloration was carried out before demineralization. Hence, an innovative global "4-in-1" process operating from hemoglobin hydrolysis to the recovery of demineralized bioactive peptides using an EDBM system with no fouling was proposed. Moving toward a circular economy, this sustainable technology has great potential for industrial hydrolysis of blood, a slaughterhouse byproduct produced in large quantities all around the world. |
| PDF |
https://doi.org/10.1021/acssuschemeng.1c04793
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