| Title | Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge |
|---|---|
| ID_Doc | 21805 |
| Authors | Lotti, T; Carretti, E; Berti, D; Martina, MR; Lubello, C; Malpei, F |
| Title | Extraction, recovery and characterization of structural extracellular polymeric substances from anammox granular sludge |
| Year | 2019 |
| Published | |
| Abstract | The composition and colloidal properties of extracellular polymeric substances (EPS) from anammox granular sludge were investigated through a complete set of spectroscopic and scattering techniques. To fully characterize EPS, we developed a robust and reproducible extraction/recovery protocol specific for anammox biofilms, based on the change of water affinity under alternated alkaline and acidic conditions, each monitored with Z-potential and dynamic light scattering analysis. This method enabled both extraction as a colloidal suspension and recovery as a solid of large amounts of EPS (0.38 +/- 0.04 and 0.21 +/- 0.02 g/g, respectively), including for the first time its structural components. The dominance of the proteinaceous fraction was revealed by all methods tested, resulting in the highest protein/carbohydrates ratio reported for biofilms applied in the wastewater sector. The abundance of proteinaceous ordered structures and in particular of cross-beta motifs was detected, indicating for the first time the presence of amyloid-like aggregates in anammox EPS, and suggesting the key role of the protein fraction in determining the mechanical properties of the parent biofilm. The robustness and reproducibility of the proposed method fill the current gap towards a reliable full-scale recovery as well as towards an accurate and meaningful investigation of anammox EPS and pave the way for further exploration of their applicative potential thus stimulating the desirable shift from the current wastewater treatment perspective towards biorefinery in a circular economy context. |
Similar Articles
| ID | Score | Article |
|---|---|---|
13353
|
Izadi, P; Izadi, P; Eldyasti, A Holistic insights into extracellular polymeric substance (EPS) in anammosx bacterial matrix and the potential sustainable biopolymer recovery: A review(2021) | |
| 21610
|
Feng, CJ; Lotti, T; Canziani, R; Lin, YM; Tagliabue, C; Malpei, F Extracellular biopolymers recovered as raw biomaterials from waste granular sludge and potential applications: A critical review(2021) | |
| 29361
|
Zeng, RG; Shi, C; Hao, LT; Huang, A; Yuan, T; Zhang, N A review of alginate-like extracellular polymers from excess sludge: Extraction, characterization, and potential application(2023) | |
| 26163
|
Lotti, T; Carretti, E; Berti, D; Montis, C; Del Buffa, S; Lubello, C; Feng, CJ; Malpei, RCC Hydrogels formed by anammox extracellular polymeric substances: structural and mechanical insights(2019) | |
| 12892
|
Oliveira, AS; Amorim, CL; Ramos, MA; Mesquita, DP; Inocêncio, P; Ferreira, EC; van Loosdrecht, M; Castro, PML Variability in the composition of extracellular polymeric substances from a full-scale aerobic granular sludge reactor treating urban wastewater(2020)Journal Of Environmental Chemical Engineering, 8.0, 5 | |
| 22241
|
Cydzik-Kwiatkowska, A Biopolymers in Aerobic Granular Sludge-Their Role in Wastewater Treatment and Possibilities of Re-Use in Line with Circular Economy(2021)Energies, 14.0, 21 |