| Title | Enzymatic hydrolysis of single-use bioplastic items by improved recombinant yeast strains |
|---|---|
| ID_Doc | 27686 |
| Authors | Myburgh, MW; Zyl, WHV; Modesti, M; Viljoen-Bloom, M; Favaro, L |
| Title | Enzymatic hydrolysis of single-use bioplastic items by improved recombinant yeast strains |
| Year | 2023 |
| Published | |
| Abstract | Single-use bioplastic items pose new challenges for a circular plastics economy as they require different processing than petroleum-based plastics items. Microbial and enzymatic recycling approaches could address some of the pitfalls created by the influx of bioplastic waste. In this study, the recombinant expression of a cutinaselike-enzyme (CLE1) was improved in the yeast Saccharomyces cerevisiae to efficiently hydrolyse several commercial single-use bioplastic items constituting blends of poly(lactic acid), poly(1,4-butylene adipate-co-terephthalate), poly(butylene succinate) and mineral fillers. The hydrolysis process was optimised in controlled bioreactor configurations to deliver substantial monomer concentrations and, ultimately, 29 to 78% weight loss. Product inhibition studies and molecular docking provided insights into potential bottlenecks of the enzymatic hydrolysis process, while FT-IR analysis showed the preferential breakdown of specific polymers in blended commercial bioplastic items. This work constitutes a step towards implementing enzymatic hydrolysis as a circular economy approach for the valorisation of end-of-life single-use bioplastic items. |
| https://doi.org/10.1016/j.biortech.2023.129908 |
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