| Title | Bioglycerol (C3) upgrading to 2,3-butanediol (C4) by cell-free extracts of Enterobacter aerogenes NCIM 2695 |
|---|---|
| ID_Doc | 27943 |
| Authors | Parate, R; Borgave, M; Dharne, M; Rode, C |
| Title | Bioglycerol (C3) upgrading to 2,3-butanediol (C4) by cell-free extracts of Enterobacter aerogenes NCIM 2695 |
| Year | 2021 |
| Published | Journal Of Chemical Technology And Biotechnology, 96.0, 5 |
| Abstract | BACKGROUND Production of biobased chemicals from renewable resources is a green starring approach that serves as a substitute to petroleum derivatives. Bioglycerol, with its growing production as a co-product of biodiesel, is an attractive low-cost feedstock for the synthesis of platform chemicals by microbial fermentation. 2,3-butanediol (2,3-BDO) is amongst the top biorefinery platform chemicals that can be produced by glycerol fermentation. RESULTS The 'Circular Economy' concept is demonstrated by converting the by-product bioglycerol using a cell-free extract of Enterobacter aerogenes NCIM 2695 (National Collection of Industrial Microorganisms, NCIM), yielding 22 g L-1 2,3-BDO, in 96 h, 98% atom economy and 0.4 g/g E factor. The cell-free bioglycerol conversion to 2,3-BDO was proved using a modified procedure for determining glycerol dehydrogenase enzyme assay by protein analysis and it was also shown to be cell-bounded. CONCLUSION Our study offers an effective utilization of the leftover material (i.e. cell-free extract) that biocatalysed C3 to C4 diol, which adds value to the overall economics of the process using only crude glycerol (C3) itself as a fermentative medium. |
Similar Articles
| ID | Score | Article |
|---|---|---|
26105
|
Sierra, W; Menéndez, P; Giordano, SR Metabolic engineered E. coli for the production of (R)-1,2-propanediol from biodiesel derived glycerol(2022)Biofuels-Uk, 13, 8 | |
| 2992
|
Costa-Gutierrez, SB; Saez, JM; Aparicio, JD; Raimondo, EE; Benimeli, CS; Polti, MA Glycerol as a substrate for actinobacteria of biotechnological interest: Advantages and perspectives in circular economy systems(2021) | |
| 25857
|
Elsayed, M; Eraky, M; Osman, AI; Wang, J; Farghali, M; Rashwan, AK; Yacoub, IH; Hanelt, D; Abomohra, A Sustainable valorization of waste glycerol into bioethanol and biodiesel through biocircular approaches: a review(2024)Environmental Chemistry Letters, 22, 2 | |
| 29560
|
Kazimierowicz, J; Debowski, M; Zielinski, M; Ignaciuk, A; Mlonek, S; Sanchez, JC The Biosynthesis of Liquid Fuels and Other Value-Added Products Based on Waste Glycerol-A Comprehensive Review and Bibliometric Analysis(2024)Energies, 17.0, 12 | |
| 12532
|
Fu, J; Wang, M; Liu, WX; Chen, T Latest Advances of Microbial Production of 2,3-Butanediol(2012)Progress In Chemistry, 24.0, 11 | |
| 14667
|
Filippousi, R; Tsouko, E; Mordini, K; Ladakis, D; Koutinas, AA; Aggelis, G; Papanikolaou, S Sustainable arabitol production by a newly isolated Debaryomyces prosopidis strain cultivated on biodiesel-derived glycerol(2022)Carbon Resources Conversion, 5, 1 | |
| 7405
|
Karmee, SK A review on preparation and applications of glycerol carbonate: Focus biocatalysis(2023) | |
| 10797
|
Chilakamarry, CR; Sakinah, AMM; Zularisam, AW; Pandey, A; Vo, DVN Technological perspectives for utilisation of waste glycerol for the production of biofuels: A review(2021) | |
| 14294
|
Angelicola, MV; Fernández, PM; Aybar, MJ; Van Nieuwenhove, CP; Figueroa, LIC; Viñarta, SC Bioconversion of commercial and crude glycerol to single-cell oils by the Antarctic yeast Rhodotorula glutinis R4 as a biodiesel feedstock(2023) | |
| 5285
|
Intasian, P; Prakinee, K; Phintha, A; Trisrivirat, D; Weeranoppanant, N; Wongnate, T; Chaiyen, P Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability(2021)Chemical Reviews, 121, 17 |