| Title | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
|---|---|
| ID_Doc | 28888 |
| Authors | Guzmán, F; Gauna, A; Roman, T; Luna, O; Alvarez, C; Pareja-Barrueto, C; Mercado, L; Albericio, F; Cárdenas, C |
| Title | Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy |
| Year | 2021 |
| Published | Molecules, 26.0, 16 |
| Abstract | Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemical features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25-30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained. |
| https://www.mdpi.com/1420-3049/26/16/5035/pdf?version=1629382207 |
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