| Title |
Self-Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed-Bed Reactors |
| ID_Doc |
7036 |
| Authors |
Peschke, T; Bitterwolf, P; Rabe, KS; Niemeyer, CM |
| Title |
Self-Immobilizing Oxidoreductases for Flow Biocatalysis in Miniaturized Packed-Bed Reactors |
| Year |
2019 |
| Published |
Chemical Engineering & Technology, 42, 10 |
| Abstract |
The industrial implementation of enzymes in flow biocatalysis microreactors is expected to be essential for the emergence of a bio-based circular economy. Major challenges concern the efficient immobilization of delicate enzymes inside miniaturized reactors without compromising their catalytic activity. We describe the exploitation of the widely used His-tag system in a microfluidic packed-bed reactor that contains ketoreductase-functionalized magnetic beads. In a continuous process, these reactors produced highly stereoselective (R)-configured alcohols (d.r. 99:1) with an average conversion of > 90 % for more than 4 days. We believe that such miniaturized flow reactors can be of great utility for future sustainable production processes. |
| PDF |
https://publikationen.bibliothek.kit.edu/1000096395/150628012
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