| Title | Multiclass ultrasound-assisted extraction, clean-up and high performance liquid chromatography-tandem mass spectrometry quantification of steroid hormone residues in compost |
|---|---|
| ID_Doc | 9506 |
| Authors | Speltini, A; Merlo, F; Maraschi, F; Bianchini, P; Mandri, A; Profumo, A |
| Title | Multiclass ultrasound-assisted extraction, clean-up and high performance liquid chromatography-tandem mass spectrometry quantification of steroid hormone residues in compost |
| Year | 2023 |
| Published | |
| Abstract | An analytical method for multiclass determination of steroid hormones in compost has been developed to fill the lack of methods for steroid residuals monitoring in this waste-derived product, increasingly produced and recycled in the circular-economy approach. The procedure simply entails an ultrasound -assisted extraction (UAE) on 300 mg compost by 3 x 2.5 mL methanol x 5 min sonication steps fol-lowed by a quick clean-up by solid-phase extraction (SPE) on the silica-based SupelcleanTMLC-NH2 that avoids use of organic solvents. The clean extract is analysed by HPLC-MS/MS achieving firm identification and quantitation of the 16 steroids, i.e., glucocorticoids, progestins, androgens, oestrogens. The analyti-cal figures of merits were assessed, viz. selectivity, sensitivity, linearity, matrix effect, trueness, precision, carry-over and robustness, in line with updated guidelines. Recovery was investigated in the concentra-tion range 15-800 ng g -1, and at the quality control levels (15, 50, 200 and 400 ng g -1) was in the range 60-120%, with inter-day precision RSDs < 20% ( n = 3). The experimental quantification limit was 15 ng g -1 for all the hormones. The method was applied to analysis of different compost samples proving to be functional to environmental monitoring.(c) 2023 Elsevier B.V. All rights reserved. |
| https://doi.org/10.1016/j.chroma.2023.463900 |
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