| Title |
Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production |
| ID_Doc |
10986 |
| Authors |
Carota, E; Petruccioli, M; D'Annibale, A; Crognale, S |
| Title |
Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production |
| Year |
2020 |
| Published |
Heliyon, 6, 9 |
| DOI |
10.1016/j.heliyon.2020.e04801 |
| Abstract |
The aqueous extraction of orange peel waste (OPW), the byproduct of the juice extraction process generated annually in massive amounts (21 Mton), yields a carbohydrate-rich liquid fraction, termed orange peel extract (OPE). Several studies highlight that the combination of glycerol, a biodiesel byproduct, with carbohydrate mixtures might boost microbial lipid production. This study performed first a shaken flask screening of 15 oleaginous yeast strains based on their growth and lipid-producing abilities on OPE- and glycerol-based media. This screening enabled the selection of R. toruloides NRRL 1091 for the assessment of the process transfer in a stirred tank reactor (STR). This assessment relied, in particular, on either single- and double-stage feeding fed-batch (SSF-FB and DSF-FB, respectively) processes where OPE served as the primary medium and nitrogen-containing glycerol-OPE mixtures as the feeding one. The continuous supply mode at low dilution rates (0.02 and 0.01 h(-1) for SSF-FB and DSF-FB, respectively) starting from the end of the exponential growth of the initial batch phase enabled the temporal extension of biomass and lipid production. The SSF-FB and DSF-FB processes attained high biomass and lipid volumetric productions (LVP) and ensured significant lipid accumulation on a dry cell basis (Y-L/X). The SSF-FB process led to LVP of 20.6 g L-1 after 104 h with volumetric productivity (r(L)) of 0.20 g L-1 h(-1) and Y-L/X of 0.80; the DSF-FB process yielded LVP, r(L) and Y-L/X values equal to 15.92 g L-1, 0.11 g L-1 h(-1) and 0.65, respectively. The fatty acid profiles of lipids from both fed-batch processes were not significantly different and resembled that of Jatropha oil, a vastly used feedstock for biodiesel production. These results suggest that OPE constitutes an excellent basis for the fed-batch production of R. toruloides lipids, and this process might afford a further option in OPW-based biorefinery. |
| Author Keywords |
Biotechnology; Microbiology; Waste treatment; Green engineering; Sustainable development; Microbial biotechnology; Biofuel; Orange peel waste; Biodiesel; Fed-batch process; Rhodosporidium toruloides; Oleaginous yeasts; Circular economy |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000579136000038 |
| WoS Category |
Multidisciplinary Sciences |
| Research Area |
Science & Technology - Other Topics |
| PDF |
http://www.cell.com/article/S2405844020316443/pdf
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