| Title |
Kinetic Modeling of the Post-consumer Poly(Ethylene Terephthalate) Hydrolysis Catalyzed by Cutinase from Humicola insolens |
| ID_Doc |
12655 |
| Authors |
Eugenio, ED; Campisano, ISP; de Castro, AM; Coelho, MAZ; Langone, MAP |
| Title |
Kinetic Modeling of the Post-consumer Poly(Ethylene Terephthalate) Hydrolysis Catalyzed by Cutinase from Humicola insolens |
| Year |
2022 |
| Published |
Journal Of Polymers And The Environment, 30.0, 4 |
| DOI |
10.1007/s10924-021-02301-4 |
| Abstract |
The search for a straightforward technology for post-consumer poly(ethylene terephthalate) (PC-PET) degradation is essential to develop a circular economy. In this context, PET hydrolases such as cutinases can be used as bioplatforms for this purpose. Humicola insolens cutinase (HiC) is a promising biocatalyst for PC-PET hydrolysis. Therefore, this work evaluated a kinetic model, and it was observed that the HiC seems not to be inhibited by any of the main PET hydrolysis products such as terephthalic acid (TPA), mono-(2-hydroxyethyl) terephthalate (MHET), and bis-(2-hydroxyethyl) terephthalate (BHET). The excellent fitting of the experimental data to a kinetic model based on enzyme-limiting conditions validates its employment for describing the enzymatic PC-PET hydrolysis using two-particle size ranges (0.075-0.250, and 0.250-0.600 mm) and temperatures (40, 50, 55, 60, 70, and 80 degrees C). The Arrhenius law provided a reliable parameter (activation energy of 98.9 +/- 2.6 kJ mol(-1)) for enzymatic hydrolysis, which compares well with reported values for chemical PET hydrolysis. The thermodynamic parameters of PC-PET hydrolysis corresponded to activation enthalpy of 96.1 +/- 3.6 kJ mol(-1) and activation entropy of 78.9 +/- 9.5 J mol(-1) K-1. Thus, the observed rate enhancement with temperature was attributed to the enthalpic contribution, and this understanding is helpful to the comprehension of enzymatic behavior in hydrolysis reaction. |
| Author Keywords |
PET biodegradation; Humicola insolens cutinase; Kinetic parameters; Heterogeneous biocatalysis |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000705801600002 |
| WoS Category |
Engineering, Environmental; Polymer Science |
| Research Area |
Engineering; Polymer Science |
| PDF |
|