| Title |
Reprogramming Filamentous fd Viruses to Capture Copper Ions |
| ID_Doc |
13650 |
| Authors |
Korkmaz, N; Usman, M; Kim, M |
| Title |
Reprogramming Filamentous fd Viruses to Capture Copper Ions |
| Year |
2024 |
| Published |
Chembiochem, 25, 13 |
| DOI |
10.1002/cbic.202400237 |
| Abstract |
C-terminal truncated variants (A, VA, NVA, ANVA, FANVA and GFANVA) of our recently identified Cu(II) specific peptide "HGFANVA" were displayed on filamentous fd phages. Wild type fd-tet and engineered virus variants were treated with 100 mM Cu(II) solution at a final phage concentration of 1011 vir/ml and 1012 vir/ml. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) imaging before Cu(II) exposure showed approximate to 6-8 nm thick filamentous virus layer formation. Cu(II) treatment resulted in aggregated bundle-like assemblies with mineral deposition. HGFANVA phage formed aggregates with an excessive mineral coverage. As the virus concentration was 10-fold decreased, nanowire-like assemblies were observed for shorter peptide variants A, NVA and ANVA. Wild type fd phages did not show any mineral formation. Energy dispersive X-ray spectroscopy (EDX) analyses revealed the presence of C and N peaks on phage organic material. Cu peak was only detected for engineered viruses. Metal ion binding of viruses was next investigated by enzyme-linked immunosorbent assay (ELISA) analyses. Engineered viruses were able to bind Cu(II) forming mineralized intertwined structures although no His (H) unit was displayed. Such genetically reprogrammed virus based biological materials can be further applied for bioremediation studies to achieve a circular economy. Filamentous fd viruses are genetically modified to display C-terminal truncated variants of the Cu(II) specific peptide ,,HGFANVA" omitting the His(H) unit. Although wild type fd phages do not result in any mineralization, engineered viruses form mineralized assemblies upon Cu(II) exposure implying the enhanced Cu(II) capturing. Such reprogrammed biological materials might be applied as environmentally friendly bioremediation agents. image |
| Author Keywords |
bioremediation; copper; fd viruses; genetic engineering; phage display |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001248881100001 |
| WoS Category |
Biochemistry & Molecular Biology; Chemistry, Medicinal |
| Research Area |
Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| PDF |
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