| Title |
Bioprospecting CAZymes repertoire of Aspergillus fumigatus for eco-friendly value-added transformations of agro-forest biomass |
| ID_Doc |
13799 |
| Authors |
Joshi, N; Grewal, J; Drewniak, L; Pranaw, K |
| Title |
Bioprospecting CAZymes repertoire of Aspergillus fumigatus for eco-friendly value-added transformations of agro-forest biomass |
| Year |
2024 |
| Published |
Biotechnology For Biofuels And Bioproducts, 17, 1 |
| DOI |
10.1186/s13068-023-02453-6 |
| Abstract |
BackgroundValorizing waste residues is crucial to reaching sustainable development goals and shifting from a linear fossil-based economy to a circular economy. Fungal cell factories, due to their versatility and robustness, are instrumental in driving the bio-transformation of waste residues. The present work isolated a potent strain, i.e., Aspergillus fumigatus (ZS_AF), from an ancient Zloty Stok gold mine, which showcased distinctive capabilities for efficient hydrolytic enzyme production from lignocellulosic wastes.ResultsThe present study optimized hydrolytic enzyme production (cellulases, xylanases, and beta-glucosidases) from pine sawdust (PSD) via solid-state fermentation using Aspergillus fumigatus (ZS_AF). The optimization, using response surface methodology (RSM), produced a twofold increase with maximal yields of 119.41 IU/gds for CMCase, 1232.23 IU/gds for xylanase, 63.19 IU/gds for beta-glucosidase, and 31.08 IU/gds for FPase. The secretome profiling validated the pivotal role of carbohydrate-active enzymes (CAZymes) and auxiliary enzymes in biomass valorization. A total of 77% of carbohydrate-active enzymes (CAZymes) were constituted by glycoside hydrolases (66%), carbohydrate esterases (9%), auxiliary activities (3%), and polysaccharide lyases (3%). The saccharification of pretreated wheat straw and PSD generated high reducing sugar yields of 675.36 mg/g and 410.15 mg/g, respectively.ConclusionThese findings highlight the significance of an efficient, synergistic, and cost-effective arsenal of fungal enzymes for lignocellulosic waste valorization and their potential to contribute to waste-to-wealth creation through solid-waste management. The utilization of Aspergillus fumigatus (ZS_AF) from an unconventional origin and optimization strategies embodies an innovative approach that holds the potential to propel current waste valorization methods forward, directing the paradigm toward improved efficiency and sustainability. |
| Author Keywords |
Lignocellulosic waste; Pine sawdust; Solid-state fermentation; Response surface methodology; Secretome analysis; Enzymatic hydrolysis |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001136264600001 |
| WoS Category |
Biotechnology & Applied Microbiology; Energy & Fuels |
| Research Area |
Biotechnology & Applied Microbiology; Energy & Fuels |
| PDF |
https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-023-02453-6
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