| Title |
Depolymerization of the polyester-polyurethane by amidase GatA250 and enhancing the production of 4,4′-methylenedianiline with cutinase LCC |
| ID_Doc |
15072 |
| Authors |
Xin, KY; Lu, JQ; Zeng, QY; Zhang, TY; Liu, JW; Zhou, J; Dong, WL; Jiang, M |
| Title |
Depolymerization of the polyester-polyurethane by amidase GatA250 and enhancing the production of 4,4′-methylenedianiline with cutinase LCC |
| Year |
2024 |
| Published |
Biotechnology Journal, 19, 4 |
| DOI |
10.1002/biot.202300723 |
| Abstract |
Polyurethane (PU) is a complex polymer synthesized from polyols and isocyanates. It contains urethane bonds that resist hydrolysis, which decreases the efficiency of biodegradation. In this study, we first expressed the amidase GatA250, and then, assessed the enzymatic characterization of GatA250 and its efficiency in degrading the polyester-PU. GatA250 degraded self-synthesized thermoplastic PU film and postconsumption foam with degradation efficiency of 8.17% and 4.29%, respectively. During the degradation, the film released 14.8 mu m 4,4 '-methylenedianiline (MDA), but 1,4-butanediol (BDO) and adipic acid (AA) were not released. Our findings indicated that GatA250 only cleaved urethane bonds in PU, and the degradation efficiency was extremely low. Hence, we introduced the cutinase LCC, which possesses hydrolytic activity on the ester bonds in PU, and then used both enzymes simultaneously to degrade the polyester-PU. The combined system (LCC-GatA250) had higher degradation efficiency for the degradation of PU film (42.2%) and foam (13.94%). The combined system also showed a 1.80 time increase in the production of the monomer MDA, and a 1.23 and 3.62 times increase in the production of AA and BDO, respectively, compared to their production recorded after treatment with only GatA250 or LCC. This study provides valuable insights into PU pollution control and also proposes applicable solutions to manage PU wastes through bio-recycling. Polyurethane is a polymer with a complex structure that contains urethane bonds as its repeating unit structure. The widespread use of PU products has led to a significant amount of plastic waste. Enzymatic depolymerization is a sustainable method for treating PU polymers, although there are few reported enzymes capable of hydrolyzing urethane bonds in PU. In this study, we introduced a combination of amidase GatA250 and cutinase LCC, which hydrolyze urethane bonds and ester bonds in PU, respectively. This approach promotes the complete depolymerization of PU and contributes to the development of a plastic circular economy. image |
| Author Keywords |
combined system; enzymatic depolymerization; polyester-based PU; PU recycling |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001202740300001 |
| WoS Category |
Biochemical Research Methods; Biotechnology & Applied Microbiology |
| Research Area |
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| PDF |
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