| Title |
Nature-inspired material binding peptides with versatile polyester affinities and binding strengths |
| ID_Doc |
17295 |
| Authors |
Blanco, FG; Machatschek, R; Keller, M; Hernández-Arriaga, AM; Godoy, MS; Tarazona, NA; Prieto, MA |
| Title |
Nature-inspired material binding peptides with versatile polyester affinities and binding strengths |
| Year |
2023 |
| Published |
|
| DOI |
10.1016/j.ijbiomac.2023.126760 |
| Abstract |
Biodegradable polyesters, such as polyhydroxyalkanoates (PHAs), are having a tremendous impact on biomedicine. However, these polymers lack functional moieties to impart functions like targeted delivery of molecules. Inspired by native GAPs, such as phasins and their polymer-binding and surfactant properties, we generated small material binding peptides (MBPs) for polyester surface functionalization using a rational approach based on amphiphilicity. Here, two peptides of 48 amino acids derived from phasins PhaF and PhaI from Pseudomonas putida, MinP and the novel-designed MinI, were assessed for their binding towards two types of PHAs, PHB and PHOH. In vivo, fluorescence studies revealed selective binding towards PHOH, whilst in vitro binding experiments using the Langmuir-Blodgett technique coupled to ellipsometry showed KD in the range of nM for all polymers and MBPs. Marked morphological changes of the polymer surface upon peptide adsorption were shown by BAM and AFM for PHOH. Moreover, both MBPs were successfully used to immobilize cargo proteins on the polymer surfaces. Altogether, this work shows that by redesigning the amphiphilicity of phasins, a high affinity but lower specificity to polyesters can be achieved in vitro. Furthermore, the MBPs demonstrated binding to PET, showing potential to bind cargo molecules also to synthetic polyesters. |
| Author Keywords |
Material binding peptides; Bacterial polyesters; Surface functionalization; Langmuir-Blodgett; Pseudomonas putida; Phasins |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001080039100001 |
| WoS Category |
Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science |
| Research Area |
Biochemistry & Molecular Biology; Chemistry; Polymer Science |
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