| Title |
Structural and conformational stability of hemocyanin from the garden snail Cornu aspersum |
| ID_Doc |
18442 |
| Authors |
Dolashki, A; Velkova, L; Voelter, W; Dolashka, P |
| Title |
Structural and conformational stability of hemocyanin from the garden snail Cornu aspersum |
| Year |
2019 |
| Published |
Zeitschrift Fur Naturforschung Section C-A Journal Of Biosciences, 74.0, 5-6 |
| DOI |
10.1515/znc-2018-0084 |
| Abstract |
Various aspects of biomedical applications of molluscan hemocyanins, associated with their immunogenic properties and antitumor activity, promoted us to perform structural studies on these glycoproteins. The stability and reassociation behavior of native Cornu aspersum hemocyanin (Call) are studied in the presence of different concentrations of Ca2+ and Mg2+ ions and pH values using electron microscopy. Higher concentrations of those ions led to a more rapid reassociation of Call, resulting in stable multidecamers with different lengths. The conformational changes of native CaH are investigated within a wide pH-temperature range by UV circular dichroism. The relatively small changes of initial [theta](lambda) indicated that many secondary structural elements are preserved, even at high temperatures above 80 degrees C, especially at neutral pH. The mechanism of thermal unfolding of Call has a complicated character, and the process is irreversible. The conformational stability of the native didecameric aggregates of Call toward various denaturants indicates that hydrophilic and polar forces stabilize the quaternary structure. For the first time, the unfolding of native CaH in water solutions in the presence of four different denaturants is investigated. The free energy of stabilization in water, Delta G(D)(H2O), was calculated in the range of 15.48 16.95 kJ mol(-1) The presented results will facilitate the further investigation of the properties and potential applications of CaH. |
| Author Keywords |
circular dichroism spectroscopy; conformational stability; Cornu aspersum hemocyanin; free energy of stabilization in water |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000466860600002 |
| WoS Category |
Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| Research Area |
Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| PDF |
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