| Title |
Optimization and validation of a quick and responsive LC-ESI-MS/MS method to evaluate tetracycline residues in processed animal proteins (PAPs) |
| ID_Doc |
21792 |
| Authors |
Morello, S; Pederiva, S; Avolio, R; Squadrone, S; Abete, MC; Marchis, D |
| Title |
Optimization and validation of a quick and responsive LC-ESI-MS/MS method to evaluate tetracycline residues in processed animal proteins (PAPs) |
| Year |
2023 |
| Published |
Analytical And Bioanalytical Chemistry, 415.0, 4 |
| DOI |
10.1007/s00216-022-04442-4 |
| Abstract |
Following the bovine spongiform encephalopathy (BSE) in 2001, processed animal proteins (PAPs) reintroduction is envisaged in non-ruminant feed thanks to their high protein content, easy availability and cost-effective characteristics. PAPs must be submitted to rendering practices, providing sterilization of products, under standardized conditions of temperature and pressure, according to Regulation (EC) No 142/2011. However, the chemical risk associated to these raw materials has been never evaluated. The aim of this study was to develop and validate a reliable liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination and quantification of tetracycline residues in PAPs at mu g kg(-1) level. The LC-MS/MS method performances were evaluated in terms of specificity, linearity (25-500 mu g kg(-1)), limit of quantitation (LOQ) (25 mu g kg(-1)), accuracy and precision (CV% < 25%), uncertainty, recovery (80-120%) and ruggedness. All the evaluated parameters fulfilled the analytical performance criteria, and the validated LC-MS/MS method fits for purpose as confirmatory method on the occurrence of residues (mu g kg(-1)) of tetracyclines in PAPs. PAPs are a powerful product which could be used both as raw materials in feed and in organic fertilizer production in a circular economy context. Therefore, the lack of regulation and control over antibiotic occurrence should be implemented to avoid a misuse and an increment of antibiotic resistance pressure over the environment and to ensure safety of the feed and food chain. |
| Author Keywords |
Processed animal proteins; Antibiotics; Tetracyclines; Validation; Liquid chromatography tandem mass spectrometry; Circular economy |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000912823700001 |
| WoS Category |
Biochemical Research Methods; Chemistry, Analytical |
| Research Area |
Biochemistry & Molecular Biology; Chemistry |
| PDF |
|