| Title |
A novel two-stage aeration strategy for Bacillus thuringiensis biopesticide production from biowaste digestate through solid-state fermentation |
| ID_Doc |
23890 |
| Authors |
Mejias, L; Estrada, M; Barrena, R; Gea, T |
| Title |
A novel two-stage aeration strategy for Bacillus thuringiensis biopesticide production from biowaste digestate through solid-state fermentation |
| Year |
2020 |
| Published |
|
| DOI |
10.1016/j.bej.2020.107644 |
| Abstract |
A novel aeration strategy has been developed at lab and bench-scale for Bacillus thuringiensis (Bt) derived biopesticides production through solid-state fermentation, using a mixture of digestate and biowaste as substrates. A Box-Behnken design was performed to select key parameters of the spore production process, being temperature and biodegradability the factors with a significant effect. Further tests confirmed the importance of oxygen content for spore production. Thus, a two-stage aeration strategy consisting of a microaeration phase followed by a high-rate aeration period was developed and tested. The production strategy was validated in a 22-L reactor using two different strains (Bt kusrtaki, Btk, and israelensis, Bti), demonstrating the robustness of the protocol. Maximum production of 1.3 x 10(8) spores g(-1) dry matter for Btk and 4 x 10(8) spores g(-1) dry matter for Bti were obtained, representing a final yield of 5 and 29 spores produced per initial CFU, respectively. |
| Author Keywords |
circular economy; digestate; biopesticide; Bacillus thuringiensis; aeration strategy |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000552131300007 |
| WoS Category |
Biotechnology & Applied Microbiology; Engineering, Chemical |
| Research Area |
Biotechnology & Applied Microbiology; Engineering |
| PDF |
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