| Title |
Sustainable biosynthesis of squalene from waste cooking oil by the yeast Yarrowia lipolytica |
| ID_Doc |
25528 |
| Authors |
Wang, SH; Sun, X; Han, YQ; Li, Z; Lu, XC; Shi, HR; Zhang, CY; Wong, A; Yu, AQ |
| Title |
Sustainable biosynthesis of squalene from waste cooking oil by the yeast Yarrowia lipolytica |
| Year |
2024 |
| Published |
|
| DOI |
10.1016/j.mec.2024.e00240 |
| Abstract |
Squalene is a highly sought-after triterpene compound in growing demand, and its production offers a promising avenue for circular economy practices. In this study, we applied metabolic engineering principles to enhance squalene production in the nonconventional yeast Yarrowia lipolytica, using waste cooking oil as a substrate. By overexpressing key enzymes in the mevalonate pathway - specifically ERG9 encoding squalene synthase, ERG20 encoding farnesyl diphosphate synthase, and HMGR encoding hydroxy-methyl-glutaryl-CoA reductase - we achieved a yield of 779.9 mg/L of squalene. Further co-overexpression of DGA1, encoding diacylglycerol acyltransferase, and CAT2, encoding carnitine acetyltransferase, in combination with prior metabolic enhancements, boosted squalene production to 1381.4 mg/L in the engineered strain Po1g17. To enhance the supply of the precursor acetyl-CoA and inhibit downstream squalene conversion, we supplemented with 6 g/L pyruvic acid and 0.7 mg/L terbinafine, resulting in an overall squalene titer of 2594.1 mg/L. These advancements underscore the potential for sustainable, large-scale squalene production using Y. lipolytica cell factories, contributing to circular economy initiatives by valorizing waste materials. |
| Author Keywords |
Squalene; Y. lipolytica; Metabolic engineering; Waste cooking oil |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Emerging Sources Citation Index (ESCI) |
| EID |
WOS:001251471600001 |
| WoS Category |
Biotechnology & Applied Microbiology |
| Research Area |
Biotechnology & Applied Microbiology |
| PDF |
https://doi.org/10.1016/j.mec.2024.e00240
|