| Title |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
| ID_Doc |
25554 |
| Authors |
Correia, J; Gudiña, EJ; Lazar, Z; Janek, T; Teixeira, JA |
| Title |
Cost-effective rhamnolipid production by Burkholderia thailandensis E264 using agro-industrial residues |
| Year |
2022 |
| Published |
Applied Microbiology And Biotechnology, 106, 22 |
| DOI |
10.1007/s00253-022-12225-1 |
| Abstract |
The agro-industrial by-products corn steep liquor (CSL) and olive mill wastewater (OMW) were evaluated as low-cost substrates for rhamnolipid production by Burkholderia thailandensis E264. In a culture medium containing CSL (7.5% (v/v)) as sole substrate, B. thailandensis E264 produced 175 mg rhamnolipid/L, which is about 1.3 times the amount produced in the standard medium, which contains glycerol, peptone, and meat extract. When the CSL medium was supplemented with OMW (10% (v/v)), rhamnolipid production further increased up to 253 mg/L in flasks and 269 mg/L in a bioreactor. Rhamnolipids produced in CSL + OMW medium reduced the surface tension up to 27.1 mN/m, with a critical micelle concentration of 51 mg/L, better than the values obtained with the standard medium (28.9 mN/m and 58 mg/L, respectively). However, rhamnolipids produced in CSL + OMW medium displayed a weak emulsifying activity when compared to those produced in the other media. Whereas di-rhamnolipid congeners represented between 90 and 95% of rhamnolipids produced by B. thailandensis E264 in CSL and the standard medium, the relative abundance of mono-rhamnolipids increased up to 55% in the culture medium containing OMW. The difference in the rhamnolipid congeners produced in each medium explains their different surface-active properties. To the best of our knowledge, this is the first report of rhamnolipid production by B. thailandensis using a culture medium containing agro-industrial by-products as sole ingredients. Furthermore, rhamnolipids produced in the different media recovered around 60% of crude oil from contaminated sand, demonstrating its potential application in the petroleum industry and bioremediation. |
| Author Keywords |
Bioremediation; Corn steep liquor; Olive mill wastewater; Circular economy; Biosurfactant; Emulsification |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000866336400001 |
| WoS Category |
Biotechnology & Applied Microbiology |
| Research Area |
Biotechnology & Applied Microbiology |
| PDF |
https://doi.org/10.1007/s00253-022-12225-1
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