| Title |
Insights on the Hypoglycemic Potential of Crocus sativus Tepal Polyphenols: An In Vitro and In Silico Study |
| ID_Doc |
26322 |
| Authors |
Bellachioma, L; Morresi, C; Albacete, A; Martínez-Melgarejo, PA; Ferretti, G; Giorgini, G; Galeazzi, R; Damiani, E; Bacchetti, T |
| Title |
Insights on the Hypoglycemic Potential of Crocus sativus Tepal Polyphenols: An In Vitro and In Silico Study |
| Year |
2023 |
| Published |
International Journal Of Molecular Sciences, 24, 11 |
| DOI |
10.3390/ijms24119213 |
| Abstract |
Post-prandial hyperglycemia typical of diabetes mellitus could be alleviated using plant-derived compounds such as polyphenols, which could influence the activities of enzymes involved in carbohydrate digestion and of intestinal glucose transporters. Here, we report on the potential anti-hyperglycemic effect of Crocus sativus tepals compared to stigmas, within the framework of valorizing these by-products of the saffron industry, since the anti-diabetic properties of saffron are well-known, but not those of its tepals. In vitro assays showed that tepal extracts (TE) had a greater inhibitory action than stigma extracts (SE) on ff-amylase activity (IC50: TE = 0.60 +/- 0.09 mg/mL; SE = 1.10 +/- 0.08 mg/mL; acarbose = 0.051 +/- 0.07) and on glucose absorption in Caco-2 differentiated cells (TE = 1.20 +/- 0.02 mg/mL; SE = 2.30 +/- 0.02 mg/mL; phlorizin = 0.23 +/- 0.01). Virtual screening performed with principal compounds from stigma and tepals of C. sativus and human pancreatic ffamylase, glucose transporter 2 (GLUT2) and sodium glucose co-transporter-1 (SGLT1) were validated via molecular docking, e.g., for human pancreatic ff-amylase, epicatechin 3-o-gallate and catechin-3o-gallate were the best scored ligands from tepals ( 9.5 kcal/mol and 9.4 kcal/mol, respectively), while sesamin and episesamin were the best scored ones from stigmas (-10.1 kcal/mol). Overall, the results point to the potential of C. sativus tepal extracts in the prevention/management of diabetes, likely due to the rich pool of phytocompounds characterized using high-resolution mass spectrometry, some of which are capable of binding and interacting with proteins involved in starch digestion and intestinal glucose transport. |
| Author Keywords |
diabetes; polyphenols; post-prandial glycemia; intestinal glucose absorption; circular economy; Crocus sativus |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001006453400001 |
| WoS Category |
Biochemistry & Molecular Biology; Chemistry, Multidisciplinary |
| Research Area |
Biochemistry & Molecular Biology; Chemistry |
| PDF |
https://www.mdpi.com/1422-0067/24/11/9213/pdf?version=1684993386
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