| Title |
Biorefinery-centric ethanol and oleochemical production employing Yarrowia lipolytica and Pichia farinosa |
| ID_Doc |
26725 |
| Authors |
Manasa, S; Tharak, A; Mohan, SV |
| Title |
Biorefinery-centric ethanol and oleochemical production employing Yarrowia lipolytica and Pichia farinosa |
| Year |
2024 |
| Published |
|
| DOI |
10.1016/j.biortech.2023.130243 |
| Abstract |
The research examined the capabilities of Yarrowia lipolytica (YL) and Pichia farinosa (PF) in converting sugars to ethanol and oleochemicals. Lipid, ethanol, protein yield and gene-expressions were analysed at different substrate concentrations (3 to 30 g/L) with glucose, food waste, and fermentation-effluent. Optimal results were obtained at 20 g/L using both synthetic carbon with 4.6 % of total lipid yield. Lauric and Caprylic acid dominance was noted in total lipid fractions. Protein accumulation (6 g/L) was observed in glucose system (20 g/L) indicating yeast strains potential as single-cell proteins (SCP). Fatty-acid desaturase (FAD12) and alcohol dehydrogenase (ADH) expressions were higher at optimum condition of YL (1.15 x 10-1, 3.8 x 10-2) and PF (5.8 x 10-2, 3.8 x 10-2) respectively. Maximum carbon reduction of 87 % depicted at best condition, aligning with metabolic yield. These findings highlights promising role of yeast as biorefinery biocatalyst. |
| Author Keywords |
Single cell oil; Biodiesel; Circular economy; Single cell protein; FAD12; Green Carbon |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001165361600001 |
| WoS Category |
Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels |
| Research Area |
Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels |
| PDF |
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