| Title |
Enrichment of halotolerant hydrogen-oxidizing bacteria and production of high-value-added chemical hydroxyectoine using a hybrid biological-inorganic system |
| ID_Doc |
26989 |
| Authors |
Feng, X; Kazama, D; He, SJ; Nakayama, H; Hayashi, T; Tokunaga, T; Sato, K; Kobayashi, H |
| Title |
Enrichment of halotolerant hydrogen-oxidizing bacteria and production of high-value-added chemical hydroxyectoine using a hybrid biological-inorganic system |
| Year |
2023 |
| Published |
|
| DOI |
10.3389/fmicb.2023.1254451 |
| Abstract |
Hybrid biological-inorganic (HBI) systems show great promise as CO2 conversion platforms combining CO2 fixation by hydrogen-oxidizing bacteria (HOB) with water splitting. Herein, halotolerant HOB were enriched using an HBI system with a high-ionic-strength medium containing 180 mM phosphate buffer to identify new biocatalysts. The reactors were inoculated with samples from saline environments and applied with a voltage of 2.0 V. Once an increase in biomass was observed with CO2 consumption, an aliquot of the medium was transferred to a new reactor. After two successive subcultures, Achromobacter xylosoxidans strain H1_3_1 and Mycolicibacterium mageritense strain H4_3_1 were isolated from the reactor media. Genome sequencing indicated the presence of genes for aerobic hydrogen-oxidizing chemolithoautotrophy and synthesis of the compatible solute hydroxyectoine in both strains. Furthermore, both strains produced hydroxyectoine in the reactors under the high-ionic-strength condition, suggesting the potential for new HBI systems using halotolerant HOB to produce high-value-added chemicals. |
| Author Keywords |
CO2 fixation; circular economy; enrichment; high salinity; hydroxyectoine; hydrogen-oxidizing bacteria; valuable products |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001066955300001 |
| WoS Category |
Microbiology |
| Research Area |
Microbiology |
| PDF |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254451/pdf
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