| Title |
Waste cooking oil and molasses for the sustainable production of extracellular lipase by Saitozyma flava |
| ID_Doc |
27761 |
| Authors |
Donzella, S; Fumagalli, A; Contente, ML; Molinari, F; Compagno, C |
| Title |
Waste cooking oil and molasses for the sustainable production of extracellular lipase by Saitozyma flava |
| Year |
2024 |
| Published |
Biotechnology And Applied Biochemistry, 71.0, 4 |
| DOI |
10.1002/bab.2570 |
| Abstract |
Organic waste valorization is one of the principal goals of the circular economy. Bioprocesses offer a promising approach to achieve this goal by employing microorganisms to convert organic feedstocks into high value products through their metabolic activities. In this study, a fermentation process for yeast cultivation and extracellular lipase production was developed by utilizing food waste. Lipases are versatile enzymes that can be applied in a wide range of industrial fields, from detergent, leather, and biodiesel production to food and beverage manufacturing. Among several oleaginous yeast species screened, Saitozyma flava was found to exhibit the highest secreted lipase activity on pNP-butyrate, pNP-caproate, and pNP-caprylate. The production medium was composed of molasses, a by-product of the sugar industry, which provided nutrients for yeast biomass formation. At the same time, waste cooking oil was employed to induce and enhance extracellular lipase production. After 48 h of process, 20 g/L of yeast biomass and 150 mU/mgdw of lipase activity were achieved, with a productivity of 3 mU/mgdw/h. The purified lipase from S. flava showed optimal performances at temperature 28(degrees)C and pH 8.0, exhibiting a specific activity of 62 U/mg when using p-NPC as substrate. |
| Author Keywords |
circular economy; fermentation; lipase production; molasses; unconventional yeasts; waste cooking oil |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001176546100001 |
| WoS Category |
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| Research Area |
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| PDF |
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bab.2570
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