| Title |
A time-course analysis of Aspergillus terreus secretomes reveals the importance of pectin-degrading enzymes to increase the digestibility of soybean meal |
| ID_Doc |
29345 |
| Authors |
Plouhinec, L; Bonnin, E; Kielbasa, M; Armengaud, J; Neugnot, V; Berrin, JG; Lafond, M |
| Title |
A time-course analysis of Aspergillus terreus secretomes reveals the importance of pectin-degrading enzymes to increase the digestibility of soybean meal |
| Year |
2024 |
| Published |
|
| DOI |
10.1128/aem.02153-23 |
| Abstract |
Considering an ever-growing global population, which hit 8 billion people in the fall of 2022, it is essential to find solutions to avoid croplands competition between human food and animal feed. Agricultural co-products such as soybean meals have become important components of the circular economy thanks to their use in animal feed. Their implementation was made possible by the addition of exogenous enzymes in the diet of monogastric animals, especially fungal carbohydrate-active enzymes (CAZymes). Here, we describe a time-course production and analysis of Aspergillus terreus secretomes for the identification of CAZymes able to enhance the digestibility of soybean meals. Functional assays revealed that the release of nutrients and the degradation of pectins in soybean meals can be tightly interconnected. Using a comparative proteomics approach, we identified several fungal pectin-degrading enzymes leading to increased assimilable nutrients in the soluble fraction of soybean meals. Our results reinforce the importance of deconstructing pectic polysaccharides in feedstuffs and contribute to sharpen our understanding of the fungal enzymatic interplays involved in pectin hydrolysis. |
| Author Keywords |
carbohydrate-active enzymes; filamentous fungi; soybean meal; pectins; rhamnogalacturonan-I; animal feed |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001295177800001 |
| WoS Category |
Biotechnology & Applied Microbiology; Microbiology |
| Research Area |
Biotechnology & Applied Microbiology; Microbiology |
| PDF |
https://doi.org/10.1128/aem.02153-23
|