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Title Spent Mushroom Substrate Hydrolysis and Utilization as Potential Alternative Feedstock for Anaerobic Co-Digestion
ID_Doc 29918
Authors Vasilakis, G; Rigos, EM; Giannakis, N; Diamantopoulou, P; Papanikolaou, S
Title Spent Mushroom Substrate Hydrolysis and Utilization as Potential Alternative Feedstock for Anaerobic Co-Digestion
Year 2023
Published Microorganisms, 11.0, 2
DOI 10.3390/microorganisms11020532
Abstract Valorization of lignocellulosic biomass, such as Spent Mushroom Substrate (SMS), as an alternative substrate for biogas production could meet the increasing demand for energy. In view of this, the present study aimed at the biotechnological valorization of SMS for biogas production. In the first part of the study, two SMS chemical pretreatment processes were investigated and subsequently combined with thermal treatment of the mentioned waste streams. The acidic chemical hydrolysate derived from the hydrothermal treatment, which yielded in the highest concentration of free sugars (approximate to 36 g/100 g dry SMS, hydrolysis yield approximate to 75% w/w of holocellulose), was used as a potential feedstock for biomethane production in a laboratory bench-scale improvised digester, and 52 L biogas/kg of volatile solids (VS) containing 65% methane were produced in a 15-day trial of anaerobic digestion. As regards the alkaline hydrolysate, it was like a pulp due to the lignocellulosic matrix disruption, without releasing additional sugars, and the biogas production was delayed for several days. The biogas yield value was 37 L/kg VS, and the methane content was 62%. Based on these results, it can be concluded that SMS can be valorized as an alternative medium employed for anaerobic digestion when pretreated with both chemical and hydrothermal hydrolysis.
Author Keywords spent mushroom substrate; chemical hydrolysis; hydrothermal process; anaerobic digestion; biofuels; biomethane; agro-industrial waste valorization; waste-to-energy; circular economy
Index Keywords Index Keywords
Document Type Other
Open Access Open Access
Source Science Citation Index Expanded (SCI-EXPANDED)
EID WOS:000940417600001
WoS Category Microbiology
Research Area Microbiology
PDF https://www.mdpi.com/2076-2607/11/2/532/pdf?version=1676878429
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