| Title |
A study on motility of the green microalgae Tetraselmis tracked by digital holography |
| ID_Doc |
62271 |
| Authors |
Cavalletti, E; Sirico, D; Miccio, L; Bianco, V; Memmolo, P; Sardo, A; Balzano, S; Ferraro, P |
| Title |
A study on motility of the green microalgae Tetraselmis tracked by digital holography |
| Year |
2022 |
| Published |
|
| DOI |
10.1109/METROSEA55331.2022.9950916 |
| Abstract |
Microalgae, eukaryotic microorganisms with a fundamental ecological role in aquatic ecosystems, have been recently suggested as promising indicators of ecosystem health and/or pollutants contamination, through the investigation of several aspects regarding their physiology and behaviour, including their locomotion and motility. Here we report the use of digital holographic microscopy as a tool to study motility in a 3D environment of the green microalgae Tetraselmis. Digital Holography (DH) is a label-free, quantitative and high resolution optical technique that allows for imaging in 3D of a biological sample, enabling fast autofocusing and recognition of the objects contained in the Field of View (FoV). In the present work DH is used in off-axis configuration to track swimming Tetraselmis cells and describe their trajectories, by measuring simultaneously the tri-dimensional paths followed by the microalgae and the full set of the kinematic parameters that describe their swimming behaviour. This technique could provide an innovative tool to visualize clearly motility in 3D of organisms under study and to better understand their behaviour in response, for example, to environmental stimuli or stressors, paving the way for their use as indicators of environmental perturbations. |
| Author Keywords |
microalgae; motility; digital holography; tracking; computational microscopy |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Conference Proceedings Citation Index - Science (CPCI-S) |
| EID |
WOS:000894276700102 |
| WoS Category |
Engineering, Ocean; Oceanography |
| Research Area |
Engineering; Oceanography |
| PDF |
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