| Title |
In vitro antioxidant and anti-inflammatory activity of an oleuropein-enriched extract obtained from olives leaves on BME-UV1 cells |
| ID_Doc |
7113 |
| Authors |
Basiricò, L; Mastrogiovanni, F; Lacetera, N; Santi, L; Bernini, R; Bernabucci, U |
| Title |
In vitro antioxidant and anti-inflammatory activity of an oleuropein-enriched extract obtained from olives leaves on BME-UV1 cells |
| Year |
2023 |
| Published |
Natural Product Research, 37, 10 |
| DOI |
10.1080/14786419.2022.2106485 |
| Abstract |
In this in vitro study, for the first time was evaluated the antioxidant and anti-inflammatory effect of an Oleuropein-enriched extract (OleE) on bovine mammary epithelial cell line (BME-UV1). OleE was obtained from olives leaves and characterized by HPLC and NMR analysis. Cell viability test indicated that OleE at concentrations of 7.8 up to 250 mu g/mL did not exert cytotoxic effect. At concentration of 31.2 up to 250 mu g/mL, a dose dependent reduction of ROS production induced by hydrogen peroxide was observed. In addition, OleE at 62.5, 125 and 250 mu g/mL showed a dose-dependent reduction in gene expression of TNF, IL1B, and IL10 after exposure to LPS. The downregulation of ROS production and cytokines expression in BME-UV1 by OleE confirmed the antioxidant and anti-inflammatory properties. In vivo experiments will be necessary for future applications of OleE as natural feed supplement in dairy cattle to reduce incidence of oxidative stress in peripartal period. |
| Author Keywords |
Oleuropein-enriched extract; olives wastes; circular economy; antioxidant activity; anti-inflammatory activity; BME-UV1 cells |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000835833900001 |
| WoS Category |
Chemistry, Applied; Chemistry, Medicinal |
| Research Area |
Chemistry; Pharmacology & Pharmacy |
| PDF |
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