| Title |
Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2 |
| ID_Doc |
7787 |
| Authors |
Baptista, SL; Cunha, JT; Romaní, A; Domingues, L |
| Title |
Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2 |
| Year |
2018 |
| Published |
|
| DOI |
10.1016/j.biortech.2018.07.068 |
| Abstract |
In this work, the industrial Saccharomyces cerevisiae PE-2 strain, presenting innate capacity for xylitol accumulation, was engineered for xylitol production by overexpression of the endogenous GRE3 gene and expression of different xylose reductases from Pichia stipitis. The best-performing GRE3-overexpressing strain was capable to produce 148.5 g/L of xylitol from high xylose-containing media, with a 0.95 g/g yield, and maintained close to maximum theoretical yields (0.89 g/g) when tested in non-detoxified corn cob hydrolysates. Furthermore, a successful integrated strategy was developed for the production of xylitol from whole slurry corn cob in a presaccharification and simultaneous saccharification and fermentation process (15% solid loading and 36 FPU) reaching xylitol yield of 0.93 g/g and a productivity of 0.54 g/L.h. This novel approach results in an intensified valorization of lignocellulosic biomass for xylitol production in a fully integrated process and represents an advance towards a circular economy. |
| Author Keywords |
Xylitol production; Industrial Saccharomyces cerevisiae PE-2; GRE3 aldose reductase; Corn cob hemicellulosic hydrolysate; Presaccharification and simultaneous; saccharification and fermentation (PSSF) |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000441876100060 |
| WoS Category |
Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels |
| Research Area |
Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels |
| PDF |
https://repositorium.sdum.uminho.pt/bitstream/1822/55390/1/document_47834_1.pdf
|