| Title |
Synergism of Endo and Exo-α-1,3-Glucanases in α-1,3-Glucan Degradation: A Kinetic Study |
| ID_Doc |
8243 |
| Authors |
Dong, Z; Zhang, P; Kralj, S; Ji, Y; Schwaneberg, U |
| Title |
Synergism of Endo and Exo-α-1,3-Glucanases in α-1,3-Glucan Degradation: A Kinetic Study |
| Year |
2024 |
| Published |
Acs Sustainable Chemistry & Engineering, 12.0, 24 |
| DOI |
10.1021/acssuschemeng.4c01469 |
| Abstract |
Biobased materials are promising alternatives to traditional fossil-derived synthetic polymers to mitigate greenhouse gas emissions and to provide end-of-life benefits addressing increasing environmental concerns. Biobased polymers with advantaged degradation and reuse characteristics have attracted increasing attention. In this work, a dual-enzyme system (combining endo alpha-1,3-glucanase Agl-ST from Streptomyces thermodiastaticus HF 3-3 and exo alpha-1,3-glucanase YgjK from Escherichia coli K12) was identified for the targeted degradation of alpha-1,3-glucan. The effects of pH, metal ions, enzyme concentration, temperature, and reaction time were investigated to assess the degradation characteristics of alpha-1,3-glucan using the synergistic enzyme system. After degradation under model conditions for 10 h, the dual-enzyme system achieved a weight loss rate of 29%, releasing 4.0 mM reducing sugar from 1% alpha-1,3-glucan. Binding behavior and degradation kinetics of alpha-1,3-glucanase on alpha-1,3-glucan were studied by a quartz crystal microbalance with dissipation monitoring. This dual-alpha-1,3-glucanase enzyme cocktail is a promising example for efficient biobased alpha-1,3-glucan polymer degradation, thereby contributing toward the concept of a circular economy. |
| Author Keywords |
alpha-1,3-glucan; alpha-1,3-glucanase; enzymatic degradation; quartz crystal microbalance withdissipation monitoring; synergistic effect |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001231837400001 |
| WoS Category |
Chemistry, Multidisciplinary; Green & Sustainable Science & Technology; Engineering, Chemical |
| Research Area |
Chemistry; Science & Technology - Other Topics; Engineering |
| PDF |
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