| Title |
Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology |
| ID_Doc |
8563 |
| Authors |
Miljkovic, MG; Davidovic, SZ; Djukic-Vukovic, AP; Ilic, MV; Simovic, MB; Rajilic-Stojanovic, MD; Dimitrijevic-Brankovic, SI |
| Title |
Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology |
| Year |
2021 |
| Published |
Hemijska Industrija, 75.0, 3 |
| DOI |
10.2298/HEMIND200710015M |
| Abstract |
Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy. |
| Author Keywords |
headspace; gas chromatography; alcoholic beverages; cosmetics |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000698039600001 |
| WoS Category |
Engineering, Chemical |
| Research Area |
Engineering |
| PDF |
http://www.doiserbia.nb.rs/ft.aspx?id=0367-598X2100015M
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