| Title |
Cloning, protein expression and biochemical characterization of Carica papaya esterase |
| ID_Doc |
8897 |
| Authors |
Reyes-Reyes, AL; Valero, F; Sandoval, G |
| Title |
Cloning, protein expression and biochemical characterization of Carica papaya esterase |
| Year |
2023 |
| Published |
|
| DOI |
10.1016/j.ejbt.2022.11.004 |
| Abstract |
Background: GDSL-like esterase/lipase proteins (GELPs) are enzymes that possess unique characteristics, they contain four invariable catalytic residues. Advances in the study of these proteins are interesting. The cloning and functional expression of a papaya esterase have not been reported. Therefore, in this work we evaluated the heterologous production of Carica papaya esterase CpEST in the yeast Komogataella phaffii (Pichia pastoris). Results: The cloning and expression of the protein was performed under the PAOX1 promoter, and productions of up to 43 AU/mL were achieved using residual glycerol from biodiesel in the batch phase and methanol for the induction phase. Enzyme activity assays determined that CpEST has a high preference for short-chain substrates (p-NP C4 and p-NP C8), and optimal activity conditions were observed at 30 degrees C and pH 10. The enzyme showed the highest stability to acetone, ethanol and tert-butanol solvents, retaining approximately 55% of its initial enzymatic activity after 1 h of exposure. Conclusions: Cloning and functional expression of papaya CpEST esterase was achieved. During fermentation, the yeasts used as a carbon source residual glycerol from biodiesel production. Based on the results obtained from the characterization of the esterase, it was found that it has a high potential for use in the bioenergy and detergent industry. How to cite: Reyes-Reyes AL, Valero F, Sandoval G. Cloning, protein expression and biochemical characterization of Carica papaya esterase. Electron J Biotechnol 2022;61. https://doi.org/10.1016/j.ejbt. 2022.11.004. (c) 2022 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Author Keywords |
Biodiesel residual glicerol; Bioenergy; Carica papaya; Circular economy; Fermentation; GDSL-like esterase; lipase proteins; Heterologous expression; Papaya esterase; Plant enzymes; Wastes recovery |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000909726400001 |
| WoS Category |
Biotechnology & Applied Microbiology |
| Research Area |
Biotechnology & Applied Microbiology |
| PDF |
https://doi.org/10.1016/j.ejbt.2022.11.004
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