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Title Slaughterhouse By-Product Valorization: Hydrolysis Degree Modification for Higher Antimicrobial Recovery by Electroseparation
ID_Doc 9598
Authors Przybylski, R; Bazinet, L; Kouach, M; Goossens, JF; Dhulster, P; Firdaous, L; Nedjar-Arroume, N
Title Slaughterhouse By-Product Valorization: Hydrolysis Degree Modification for Higher Antimicrobial Recovery by Electroseparation
Year 2021
Published Waste And Biomass Valorization, 12.0, 4
DOI 10.1007/s12649-020-01154-7
Abstract In the actual context of food safety and circular economy, the separation of the alpha 137-141 antimicrobial peptide (TSKYR, 653 Da) coming from the hydrolysis of bovine hemoglobin, a non-valorized slaughterhouse by-product, and its reuse as a preservative agent for food products during storage would be of major interest. However, depending on the degree of hydrolysis (DH) of the bovine hemoglobin, the TSKYR peptide environment into the hydrolysate will be different and would impact its recovery yield by electrodialysis with ultrafiltration membranes (EDUF), a hybrid and eco-friendly technology. In this context, five DHs (3, 5, 10, 13 and 18%) were investigated to study the influence of the peptide populations on the alpha 137-141 selective separation by EDUF. It appeared from these results that the most appropriate DH was of 5% since a lower population of peptides between 500 and 1000 Da was present and the subunits of hemoglobin were already digested. Hence, an enrichment factor of about 13 folds for the alpha 137-141 was obtained in comparison with the initial hydrolysate. At our knowledge, it was the first time that the effect of the hydrolysis degree of a protein is demonstrated to impact preferentially the separation of a specific peptide by EDUF. [GRAPHICS] .
Author Keywords Antimicrobial peptide; Electrodialysis; Filtration membrane; Preservative; Valorization
Index Keywords Index Keywords
Document Type Other
Open Access Open Access
Source Science Citation Index Expanded (SCI-EXPANDED)
EID WOS:000547220500003
WoS Category Environmental Sciences
Research Area Environmental Sciences & Ecology
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