| Title | Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil |
|---|---|
| ID_Doc | 29424 |
| Authors | Li, Y; Cheng, ZZ; Zhao, CL; Gao, C; Song, W; Liu, LM; Chen, XL |
| Title | Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil |
| Year | 2021 |
| Published | Acs Synthetic Biology, 10.0, 8 |
| Abstract | The recycle and reutilization of food wastes is a promising alternative for supporting and facilitating circular economy. However, engineering industrially relevant model organisms to use food wastes as their sole carbon source has remained an outstanding challenge so far. Here, we reprogrammed Escherichia coli metabolism using modular pathway engineering followed by laboratory adaptive evolution to establish a strain that can efficiently utilize waste cooking oil (WCO) as the sole carbon source to produce monomers of bioplastics, namely, medium-chain alpha,omega-dicarboxylic acids (MCDCAs). First, the biosynthetic pathway of MCDCAs was designed and rewired by modifying the beta-oxidation pathway and introducing an omega-oxidation pathway. Then, metabolic engineering and laboratory adaptive evolution were applied for improving the pathway efficiency of fatty acids utilization. Finally, the engineered strain E. coli AA0306 was able to produce 15.26 g/L MCDCAs with WCO as the sole carbon source. This study provides an economically attractive strategy for biomanufacturing bioplastics from food wastes, which has a great potentiality to be developed as a wide range of enabling biotechnologies for achieving green revolution. |
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