| Title |
Accelerated solvent extraction of red onion peel extract and its antimicrobial, antibiofilm, and quorum-sensing inhibition activities against Listeria monocytogenes and Chromobacterium violaceum |
| ID_Doc |
10716 |
| Authors |
Dhowlaghar, N; Dhanani, T; Pillai, SS; Patil, BS |
| Title |
Accelerated solvent extraction of red onion peel extract and its antimicrobial, antibiofilm, and quorum-sensing inhibition activities against Listeria monocytogenes and Chromobacterium violaceum |
| Year |
2023 |
| Published |
|
| DOI |
10.1016/j.fbio.2023.102649 |
| Abstract |
In this study, we used accelerated solvent extraction (ASE) to extract flavonoids and anthocyanins from dried red onion peels, producing red onion peel extract (ROPE). High-performance liquid chromatography analysis of ROPE obtained using ASE identified important flavonols and anthocyanins. Total flavonoid and anthocyanin contents measured from ROPE were 459.15 mg/g +/- 16.45 (DW) and 33.14 mg/g +/- 0.99 (DW), respectively. ROPE antimicrobial activity against L. monocytogenes showed strain-specific effects, where the minimum inhibitory concentration (MIC) for V7 was two-fold greater than for EGD and Scott A (i.e., 25.82 vs. 12.91 mg quercetin-3, 4 '-diglucoside equivalents/gram [mg QdGE/g] ROPE). ROPE significantly inhibited (P < 0.05) biofilm formation by all three strains at sub-inhibitory concentrations (0.40-6.45 mg QdGE/g) in a concentration-dependent manner. Pre-formed biofilms of mixed L. monocytogenes strains on stainless steel were inactivated after 48 h at 22 C only with higher concentrations of ROPE (25.82-56.63 mg QdGE/g). Sub -inhibitory concentrations of ROPE also inhibited L. monocytogenes motility and suppressed violacein pigment production in Chromobacterium violaceum ATCC 12472, suggesting that ROPE inhibited quorum sensing. Therefore, our data indicate that red onion peel waste has potential as a source of natural antibacterial com-pounds as an approach to food safety in the circular economy. |
| Author Keywords |
Listeria monocytogenes; Red onion peel; Biofilm inhibition; Subinhibitory concentrations; Accelerated solvent extraction; Quorum sensing; Chromobacterium violaceum |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:000998862900001 |
| WoS Category |
Food Science & Technology |
| Research Area |
Food Science & Technology |
| PDF |
http://manuscript.elsevier.com/S2212429223003000/pdf/S2212429223003000.pdf
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