| Title |
A model-driven approach to upcycling recalcitrant feedstocks in Pseudomonas putida by decoupling PHA production from nutrient limitation |
| ID_Doc |
12769 |
| Authors |
Manoli, MT; Gargantilla-Becerra, A; Sánchez, CD; Rivero-Buceta, V; Prieto, MA; Nogales, J |
| Title |
A model-driven approach to upcycling recalcitrant feedstocks in Pseudomonas putida by decoupling PHA production from nutrient limitation |
| Year |
2024 |
| Published |
Cell Reports, 43.0, 4 |
| DOI |
10.1016/j.celrep.2024.113979 |
| Abstract |
Bacterial polyhydroxyalkanoates (PHAs) have emerged as promising eco-friendly alternatives to petroleumbased plastics since they are synthesized from renewable resources and offer exceptional properties. However, their production is limited to the stationary growth phase under nutrient -limited conditions, requiring customized strategies and costly two-phase bioprocesses. In this study, we tackle these challenges by employing a model -driven approach to reroute carbon flux and remove regulatory constraints using synthetic biology. We construct a collection of Pseudomonas putida -overproducing strains at the expense of plastics and lignin -related compounds using growth -coupling approaches. PHA production was successfully achieved during growth phase, resulting in the production of up to 46% PHA/cell dry weight while maintaining a balanced carbon -to -nitrogen ratio. Our strains are additionally validated under an upcycling scenario using enzymatically hydrolyzed polyethylene terephthalate as a feedstock. These findings have the potential to revolutionize PHA production and address the global plastic crisis by overcoming the complexities of traditional PHA production bioprocesses. |
| Author Keywords |
|
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001227083200001 |
| WoS Category |
Cell Biology |
| Research Area |
Cell Biology |
| PDF |
http://www.cell.com/article/S2211124724003073/pdf
|