| Title |
Antibacterial Activity Of Combined Nanodiamonds And Snail Fractions With Biocompounds With Mw Below 10 Kda And Above 30 Kda |
| ID_Doc |
19198 |
| Authors |
Daskalova, E; Belouhova, M; Zheleva, N; Topalova, Y; Velkova, L; Dolashki, A; Dolashka, P |
| Title |
Antibacterial Activity Of Combined Nanodiamonds And Snail Fractions With Biocompounds With Mw Below 10 Kda And Above 30 Kda |
| Year |
2023 |
| Published |
Comptes Rendus De L Academie Bulgare Des Sciences, 76.0, 1 |
| DOI |
10.7546/CRABS.2023.01.04 |
| Abstract |
The use of nanomaterials for enhancement of the antibacterial activity of different compounds is a new approach contributing to the efforts of over-coming the emerging antibiotics resistance. This study explores the combined antibacterial activity of nanodiamonds and snail peptides against Brevibacillus laterosporus BT 271. Two peptide/protein fractions from Cornu aspersum mu-cus were used - with molecular weight (MW) below 10 kDa and with MW above 30 kDa. Four types of nanodiamonds (ND) were tested (positively charged, sin-gle digit and two types of detonation ND). Peptide fractions were characterized by using MALDI-TOF. The fraction with MW below 10 kDa consisted of many different peptides, while the fraction with proteins above 30 kDa showed pro-teins at m/z 26879.2 Da, 33903.11 Da, 43583.81 Da, 53986.81 Da, 79091.82 Da, 100650.42 Da, and 141 550.82 Da. When mixture peptides+ND was ap-plied directly on the bacterial culture, the nanodiamonds showed enhanced antibacterial effect from 8% up to 42%. The combination of nanodiamonds and the mucus fraction with MW above 30 kDa had strongest bactericidal ef-fect (1593.04 mm2/mgP*mu L). The results demonstrated that ND have potential to enhance the antibacterial activity of the snail peptides but also highlighted that their effects should be carefully studied. |
| Author Keywords |
nanodiamonds; Cornu aspersum; peptides; antibacterial ac-tivity; Brevibacillus laterosporus |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001010638200004 |
| WoS Category |
Multidisciplinary Sciences |
| Research Area |
Science & Technology - Other Topics |
| PDF |
http://proceedings.bas.bg/index.php/cr/article/download/231/225
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