| Title |
Enzymatic hydrolysis of single-use bioplastic items by improved recombinant yeast strains |
| ID_Doc |
27686 |
| Authors |
Myburgh, MW; Zyl, WHV; Modesti, M; Viljoen-Bloom, M; Favaro, L |
| Title |
Enzymatic hydrolysis of single-use bioplastic items by improved recombinant yeast strains |
| Year |
2023 |
| Published |
|
| DOI |
10.1016/j.biortech.2023.129908 |
| Abstract |
Single-use bioplastic items pose new challenges for a circular plastics economy as they require different processing than petroleum-based plastics items. Microbial and enzymatic recycling approaches could address some of the pitfalls created by the influx of bioplastic waste. In this study, the recombinant expression of a cutinaselike-enzyme (CLE1) was improved in the yeast Saccharomyces cerevisiae to efficiently hydrolyse several commercial single-use bioplastic items constituting blends of poly(lactic acid), poly(1,4-butylene adipate-co-terephthalate), poly(butylene succinate) and mineral fillers. The hydrolysis process was optimised in controlled bioreactor configurations to deliver substantial monomer concentrations and, ultimately, 29 to 78% weight loss. Product inhibition studies and molecular docking provided insights into potential bottlenecks of the enzymatic hydrolysis process, while FT-IR analysis showed the preferential breakdown of specific polymers in blended commercial bioplastic items. This work constitutes a step towards implementing enzymatic hydrolysis as a circular economy approach for the valorisation of end-of-life single-use bioplastic items. |
| Author Keywords |
Bioplastic waste; Single-use bioplastics; Recycling; Enzyme hydrolysis; Value-added products; Circular economy |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001102446300001 |
| WoS Category |
Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels |
| Research Area |
Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels |
| PDF |
https://doi.org/10.1016/j.biortech.2023.129908
|