| Title |
Anaerobic acidification of pressed sugar beet pulp for mcl-polyhydroxyalkanoates fermentation |
| ID_Doc |
9468 |
| Authors |
Kacanski, M; Knoll, L; Nussbaumer, M; Neureiter, M; Drosg, B |
| Title |
Anaerobic acidification of pressed sugar beet pulp for mcl-polyhydroxyalkanoates fermentation |
| Year |
2023 |
| Published |
|
| DOI |
10.1016/j.procbio.2023.06.019 |
| Abstract |
Anaerobic acidification of pressed sugar beet pulp (PSBP) is a promising strategy for the transition towards a circular economy. In this work, volatile fatty acids were produced by anaerobic acidification of PSBP and subsequently converted to mcl-polyhydroxyalkanoates. The results point to mesophilic acidification as superior to thermophilic one. At the same time, the pH regulated at the value of 6.0 showed a decisive advantage over both the pH of 7.0 and the lack of pH regulation. Furthermore, the conditions with a hydraulic retention time (HRT) of 10 days significantly outperformed those with an HRT of 6 days. The best-performing process (mesophilic, pH controlled at 6, HRT of 10 days) was successfully scaled up to a 250 L reactor, reaching a volatile fatty acid (VFA) concentration of up to 27.8 g L-1. Finally, the produced VFA were investigated as feedstock for mcl-PHA producers, Pseudomonas citronellolis and Pseudomonas putida. Both strains grew and produced PHA successfully, with P. citronellolis reaching a biomass of 15.6 g L-1 with 38% of mcl-PHA, while P. putida grew to 15.2 g L-1 with a polymer content of 31%. This study proves that acidified PSBP is a valuable feedstock for mcl-PHA production and an important approach to developing biorefineries. |
| Author Keywords |
Anaerobic digestion; Biopolymer; Acidification; Volatile fatty acids; Polyhydroxyalkanoates; Sugar industry residue |
| Index Keywords |
Index Keywords |
| Document Type |
Other |
| Open Access |
Open Access |
| Source |
Science Citation Index Expanded (SCI-EXPANDED) |
| EID |
WOS:001042256500001 |
| WoS Category |
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical |
| Research Area |
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering |
| PDF |
https://doi.org/10.1016/j.procbio.2023.06.019
|